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KMID : 0379119870150040250
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Korean Journal of Mycology
1987 Volume.15 No. 4 p.250 ~ p.253
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Transfer of Isolated Nuclei from Pleurotus florida into Protoplasts of Pleurotus ostreatus
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박용환/Park, Yong Hwan
신평균/장권열/유영복/유창협/Shin, Pyung Gyun/Chang, Kwon Yawl/Yoo, Young Bok/You, Chang Hyun
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Abstract
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The transfer of the isolated nuclei from P. Florida into protoplasts of P. ostreatus was induced with polyethylene glycol and CaCl₂. Three types of transfer products of nuclei were obtained when transferred to MMM. Type 1 colonies were more vigroualy growing mycelium and stable on MCM. One of the type 1 colonies, appeared segregation on MCM plus benomyl. The mycelium did not form clamp connection. These results suggest that type 1 colonies were nuclear hybrids or allodiploids. Type 2 was main products of nuclear transfer. The mycelium formed clamp connection and fertile on sawdust media. Type 3 was very slow growing or non-viable colonies after debris of nuclei or chromosomes transfer into recipient protoplasts.
Isozyme pattern of esterase in type 1 produced a new band. Type 2 and type 3 could be characterized by parental bands.
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KEYWORD
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